Determination of Carbapenemase Production by BD Phoenix CPO Method in Karbapenem Resistant Klebsiella pneumoniae and Escherichia coli Isolates

Aim:Carbapenemase-producing organisms are Gram-negative bacteria that resistant to most, sometimes all, antibiotics with their carbapenemase enzymes. Rapid and accurate detection of carbapenemases is essential for the early prevention nosocomial infections.
 Material Method:The study included 72 isolates, 64 containing Oxa-48, 6 NDM, 2 NDM+Oxa-48 16 isolates without carbapenemase, isolated from various clinical samples sent bacteriology unit ……. University Hospital Central Laboratory between January 2020 December 2020. Carbapenemase enzyme groups were investigated in BD Phoenix CPO Detect (Becton Dickinson, USA) kit.
 Results:The compatibility test PCR was 95.3% Oxa-48 carrying 100% those NDM gene. The negative predictive rate 88.8% all 83.3% K. pneumoniae 100.0% E. coli isolates.
 Conclusion:The usage test, which can detect enzymes , routine diagnostic laboratories will contribute diagnosis correct treatment bacteria.